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Purification was performed using Ni-NTA agarose (Qiagen) as the matrix, with 20 mM sodium phosphate, pH 7.4, 500 mM sodium chloride, and 30 mM (wash) or 250 mM (elution) imidazole as buffers

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Cyclophosphamide (CTX), ara-C and topotecan (TOP)(CAT) in patients (pts) with refractory or relapsed acute leukemias and transformed chronic myelogenous leukemia (CML)

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