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For each z-slice, a relative signal was obtained by calculating the ratio intensity (mean gray value) for Ubx signal (488 nm) relative to the DAPI signal in T2-T3 segments (which only expressed ectopic Ubx protein) using the same region of interest (ROI)

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Avoid strenuous activities and heavy lifting to prevent further soreness and strain, allowing your arm to recover more quickly

This is the most significant limitation No statistical significance testing: The paper does not report p-values or statistical tests

These compounds derive from a structure-based screening of 900,000 compounds from ChemBridge Hit2Lead library, followed by CB-27 shape similarity screening