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The supernatant was serially diluted and used (in theory, the final concentration of the corresponding E-64 analogs in the mixture was estimated to be less than 100 nM after serial dilution) for the cathepsin B inhibition assay
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Researchers working with the 20mg vial will need to pay particular attention to reconstitution ratios since larger quantities of peptide require proportionally adjusted volumes of bacteriostatic water
