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Based on their biogenesis and size, EVs are classified into three main subtypes: Exos (30150 nm in diameter), microvesicles (1501000 nm in diameter), and apoptotic bodies (502000 nm in diameter) (recently reviewed [54,55,56]

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freeze for extended storage stability

To show the outlines of acinar cells, we used Alexa Fluor 488 conjugated Wheat Germ Agglutinin (1:200) as counterstaining (W11261, Thermo Fisher, Waltham, MA, USA)

BV possesses anticancer, antimicrobial, and antiviral activities (Gajski et al., 2024)

In summary, time interval between blood withdrawal and freezing varied from 11 to 32 hours