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4.2 Detailed expandable studies Study 1: Imai & Guarente 2014, foundational review article Citation: Imai S., Guarente L

Standard readouts include histological scoring of tissue sections, immunohistochemical staining for repair-associated markers, and quantitative PCR for growth factor gene expression

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Pregnancy and breastfeeding considerations Tirzepatide is contraindicated during pregnancy and should be discontinued at least 2 months before planned conception due to the long washout period