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The structural integrity of this cartilage is maintained by specialized cells called chondrocytes, which continuously synthesize and remodel the extracellular matrix (ECM)a dense, complex network composed primarily of water, collagen fibers, and large molecules known as proteoglycans

Following the inhibition of ceramide biosynthesis, several mechanisms would be correlated with oxidative stress and apoptosis due to immunolocalization of the lower doses of FB 1 in specific targets, such as mitochondria and nucleus (Figure 1) (101)

For quantitative analyses, ratio of cells positive for nuclear -catenin staining to total number of cells was counted as pixel intensity ratio positive for nuclear localization using Image J (NIH, Bethesda, MD, USA)

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