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In the lower clamp of the machine, an aluminum block was attached, and a 50 N force was applied to the aluminum block-clamp assembly to eliminate any slippage in the lower assembly

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Thus, the spectrum to consider is expansive, including Glu from GLP-1, Asp similar to Glu from GIP, Ala, and even Leu/Ile/Val (forming a hydrophobic area along with Phe 22 )