As noted by the Alzheimers Drug Discovery Foundation (ADDF), the bulk of research involving epithalamin and epithalon has been conducted in Russia (or formerly, the Soviet Union) and has not been subject to independent confirmation
Molecular Characteristics Complete Specifications: CAS Registry Number: NOT ASSIGNED (synthetic modified peptide) Molecular Weight: 431.40 Da (42 Da higher than Epitalon due to acetyl group) Molecular Formula: CHNO Amino Acid Sequence: N-Ac-Ala-Glu-Asp-Gly (acetylated AEDG) Modification: N-terminal acetylation (acetyl group: COCH) Peptide Classification: Synthetic modified tetrapeptide Appearance: White to off-white lyophilized powder Solubility: Water, bacteriostatic water, phosphate buffered saline The N-acetyl modification adds 42 Da to the molecular weight compared to unmodified Epitalon (390.35 Da)
Rushworth, G
PMID:33567185 Davies, M., et al
To reach their target area through digestion route makes these pills have less effective absorption rates hence delaying time taken for them to become active
For the solubility test, cell pellet from 250 ml (2 10 12 cells) culture was harvested and 1250 L of a lysis and solubilization buffer containing 50 mM TrisCl pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 1% Triton-X 100, 300 mM NaCl, 1 mM PMSF and 500 L BugBuster Protein Extraction Reagent was added, maintaining the ratio of cells to buffer as ~1.6 10 12 cells/ml of buffer
