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Step 4: Deliver the dose

20 ng of cDNA was used per qPCR reaction using Powerup SYBR green master mix (Thermo Fisher Scientific) and gene specific primers according to manufacturers instructions on a Quantstudio 3 Real-Time PCR system (Thermo Fisher Scientific)

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Thwaites, DT, Anderson, CMH

481 Neurodegeneration and abnormal nervous tissue development is, arguably, triggered by the invasion of amniotic fluid into the developing spinal cord and adding to the mechanical trauma