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Cells for cell-cycle analysis were stained with PI using DNA labelling solution kit (Cytognos, Spain) following the protocol, and were analysed by FACScan

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Demand third-party purity verification via HPLC (high-performance liquid chromatography) and mass spectrometry with every batch, documented in a certificate of analysis (COA) that includes peptide content percentage, endotoxin levels, and sterility testing results

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