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The 2021 STEP 4 trial, published in JAMA, showed that people who continued semaglutide after an initial 20-week run-in kept losing weight, while those who switched to placebo regained nearly 7% of their body weight, confirming that ongoing treatment is key to maintaining results

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Assay of GST activity The GST assay reaction mixture contained in 1 ml total volume of 0.1 M K-phosphate buffer, pH 6.5, 1 mM 1-chloro-2,4-dinitrobenzene (CDNB), 1 mM GSH, and enzyme solution

The total bank is larger because it is serving multiple purposes