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10.1002/mc.20248 14 BarriosC

Vitamin K is a group of fat-soluble compounds, essential for the post-translational conversion of protein-bound glutamates into -carboxyglutamates in various target proteins

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The flow-through fraction containing biotinylated TGP Avi (free of His-tag) was loaded onto a Superdex 200 increase 10/300 GL column pre-equilibrated with buffer containing 150 mM NaCl, 20 mM Tris-HCl pH 8.0 to remove ATP and biotin