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Subsequently, the cells were stained with calcein AM and propidium iodide (PI) (C2015M, Beyotime, China) (30 min at 37 C in the dark)

10.1007/s10528-007-9129-3

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A suggested mechanism involves prolonged GIPR agonism causing receptor degradation, reducing sustained GIP signalling and, when combined with GLP-1R agonism, causing increased weight loss ( The GLP-1R has itself several LoF variants including R380C, shown by two independent groups to have normal surface expression but reduced affinity for Exenatide (Wootten et al., 2016

Targeted outcomes: Enhanced cellular proliferation and satellite cell activation at low nanomolar concentrations

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