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In laboratory animals, the forced overexpression of the TKT gene promotes the development of MAFLD as well as the advancement of MAFLD to the more severe state referred to as metabolic dysfunction-associated steatohepatitis (MASH)

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Computer-aided design (CAD) of Mn(II) complexes: Superoxide dismutase mimetics with catalytic activity exceeding the native enzyme

Materials and Methods Expression and purification The FhGST-S1 was cloned into pET23a (Novagen) as described previously 11

2021;2:72035.e724

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat