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Analysis of subgroups or subsets If sufficient data are available, subgroup analysis will be performed by type of Se supplementation

Western blot analysis RIPA Lysis Buffer (TransGen, Beijing, China) was used to extract total protein from tissues/fibroblasts and a Nuclear and Cytoplasmic Protein Extraction Kit (TransGen) was used to extract the protein from nucleus and cytoplasm of fibroblasts

The gene High Mobility Group AT-Hook 2 ( HMGA2

10.1111/j.1572-0241.1992.tb07323.x 96 ShinS

Importantly, no severe treatment-related adverse effects were documented across any trial

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