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NAPQI is rapidly conjugated to glutathione, limiting its toxicity while depleting cellular glutathione stores 14,30

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The first step in the synthesis of GSH is the formation of -glutamyl-cysteine (GluCys) from the constituent amino acids glutamate and cysteine by the enzyme -glutamyl-cysteine synthetase (GCS, also called glutamate cysteine ligase (GCL))

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421 Specifically, dysfunction within the ventrolateral periaqueductal gray (vlPAG)-mediated descending pain modulatory system has been reported in patients with PDPN

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