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[1] [2] The MHRA and prescribing information provide comprehensive guidance on adverse effect profiles, though hot flushes are not listed among the established side effects in the official product literature

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Human phospho-site mutant GLP-1R construct generation SNAP/FLAG-tagged human GLP-1R wild-type (WT) plasmid (Cisbio), subsequently referred to as SNAP/FLAG-hGLP-1R, was altered by site-directed mutagenesis with the PfuUltra II Fusion High-fidelity DNA Polymerase (Agilent) as per the manufacturers protocol to modify serine to alanine doublets at positions 441/442, 444/445, and 451/452 of the receptor to generate individual serine doublet mutants (referred to as S441/2A, S444/5A and S451/2A)