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The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L 1 NaCl at 12 C) and quenched in cold methanol/water (1:1 v/v) at 30 C as described by Smart et al

However, it is also more expensive and may not be covered by all insurance plans

Providers can acknowledge the emerging data while emphasizing that definitive clinical benefits have not yet been established

Nrf2: Nuclear Factor-Erythroid 2-Related Factor 2

Abbreviations AC, Adenylyl Cyclase