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Cells were then placed on ice, washed with cold HBSS, and incubated for 10 minutes in cold acetic acid + 150 mM NaCl buffer, pH 2.9, to strip surface ligand

As an anti-inflammatory agent, NAC prevents the release of pro-inflammatory cytokines in the initial state of immune proliferation in disease progression, and this mechanism is based on the inhibition of the activities of lipopolysaccharides

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Cardiorenal Med 3:200207

Transcriptomic analysis by RNA sequencing Total RNA was isolated using an RNeasy mini kit (Qiagen, #74106) according to the manufacturers protocol

Enhances metabolic function by targeting GLP-1, GIP, and glucagon receptors simultaneously