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The fluorescence intensities had a fine linear to the logarithm of GSH concentrations in the range from 10 10 M to 1.0 10 7 M in Fig

Journal of Molecular Medicine (Berlin), 89 (3), 205212

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First, a special amino acid in the enzymes active siteoften selenocysteine in key isoformsgets oxidized by the peroxide, forming an intermediate called selenenic acid

Because FOXO4-DRIs mechanism depends on disrupting the FOXO4p53 interaction, and because this interaction is only operative in cells with elevated FOXO4 expression, the compound preferentially targets the senescent cell population leaving FOXO4-low non-senescent cells largely unaffected