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10.1126/scitranslmed.3002847 237 OyewoleA

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The results of Western blotting revealed that the ratio of p-AMPK/total AMPK in the GLUT4-siRNA group was significantly increased compared to the control (Figure 3C), which indicates that the downregulation of GLUT4 affects the stability of energy metabolism in mouse EECs by insufficient glucose intake

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Mistake 2: Using wrong water Bacteriostatic water only