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The parameters of the QTRAP 5500 ESI source were as follows: Positive Ion Mode: Source Temperature 550C, IonSource Gas1 (GAS1): 40, Ion Source Gas2 (GAS2): 50, Curtain Gas (CUR): 35, Ion Spray Voltage Floating (ISVF) 5,500 V

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NIH/3T3 cells were seeded on 6-well plates (2 10 4 cells per well) and cultured for 24 h