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After 48 h, the cells were washed with PBS (Gibco) and incubated in the assay buffer (HBSS supplemented with Ca 2+ and Mg 2+ (Gibco), 10 mM HEPES (Gibco), 0.1% Pluronic F-68 (Gibco) and 500 M IBMX (Sigma), pH 7.4) with different concentrations of GLP-1 (0 M to 1 M) in the presence (100 nM and 1000 nM) or absence of Fab7F38 at 37 C for 30 min

The medication is given as a once-weekly injection

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However, the gas produced by fermentation of inulin may aggravate the gastrointestinal symptoms of patients, thus limiting its beneficial effects (155)

Patients should follow their clinician's recommendations closely and seek immediate medical attention if they experience severe side effects