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As NAC and GSH are thiol group (-SH)-containing antioxidants, apart from directly neutralizing ROS, they also provide precursors for endogenous GSH synthesis including cysteine, glutamine and glycine under the regulation of NRF2 (Nuclear Factor, Erythroid 2 like 2), the master gene regulator responding to cellular oxidative stress, and GCLC (Glutamate-cysteine ligase catalytic subunit), and the first rate-limited enzyme in cellular GSH biosynthesis [17, 18]