For samples derived from media containing 13 C glucose or 13 C glutamine, methoximation was followed by silylation by adding 80 l of MTBSTFA (with 1% TBDMS, Thermo) and incubation for 60 min at 70 C
Stanley CA, DeLeeuw S, Coates PM, et al
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This technique deposits the peptide into the fatty layer beneath the skin, from which it gradually absorbs into circulation
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Overexpression of GSTP1 resulted in a two-fold increase in GSH-conjugation of the QIs, attenuated QI-induced cytotoxicity especially under GSH-depletion condition, abolished QIs-induced apoptosis but did not significantly inhibit the activation of the ER stress response