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Ideally, a temperature between 20C and 25C (68F and 77F)

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Shaking can shear and destroy the peptide chains

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Vitamin B12 Deficiency: Insufficient dietary intake of B12 (common in vegetarians and vegans), Malabsorption due to conditions like pernicious anemia or gastrointestinal disorders (e.g., Crohns disease), Certain medications that interfere with B12 absorption can lead to deficiency

The benzyl alcohol helps maintain the solution's sterility, preventing contamination each time the rubber stopper is punctured