Electron microscopy MIN6B1-SNAP-GLP-1R cells cultured on Thermanox coverslips (Agar Scientific) were labeled with 2 M cleavable SNAP-Surface biotin probe in HEPES-bicarbonate buffer plus 3 mM glucose and 1% BSA, followed by incubation with 5 g ml 1 NaN 3 -free Alexa Fluor 488 Streptavidin, 10 nm colloidal gold conjugate (Molecular Probes) and stimulation with 100 nM agonist in buffer plus 11 mM glucose
Also, there are nerves in the gut which can sense GLP-1 and signal back to the brain, so the picture is a lot more complicated and more research is being carried out into the way GLP-1 and GLP-1 mimetics work
While this is its primary role in diabetes management, stable blood sugar can also contribute to better appetite control and reduced cravings
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