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The eluted fractions were analyzed by SDSPAGE, and protein bands were visualized using a ChemiDoc imaging system (Bio-Rad)

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Visible improvements typically become apparent after 3-6 months of consistent supplementation, as hair growth cycles require time to respond to nutritional changes

doi: 10.1155/2014/426951 118 RossCMorrissAKhairyMKhalafYBraudePCoomarasamyAet al

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Altogether, these findings enhance our understanding of the molecular basis for acyl-ghrelin recognition and activation of the ghrelin receptor and provide a framework for the drug design targeting the ghrelin receptor