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RNA-Sequencing RNA was extracted from HCC cells cultured in DMEM + 10% FS for 24 hours using a Qiagen RNeasy Plus Mini Kit (Qiagen #74134) with an additional on column DNA digestion using Qiagen TURBO DNase (Qiagen #AM2238) based on manufacturer protocols

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Retatrutide is currently in phase 3 clinical trials, and the timeline for what happens next depends on study completion and regulatory review