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RTqPCR Total RNA was isolated using the RNeasy Mini Kit (Qiagen, 74104) and reverse-transcribed with a high-capacity RNA-to-cDNA kit (Vazyme, R323-01)

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Identification and prediction of molecular subtypes of atherosclerosis based on m6A immune cell infiltration

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Finally, heterogeneity in ascent profiles, altitude reached, duration of exposure, and supplementation protocols, quantified by the I 2 statistic and addressed using a random-effects model, may have contributed to variability across studies and constrained further stratified or sensitivity analyses