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For Fos immunostaining, anti-Fos antibody (ab190289, Abcam, Cambridge, UK) was diluted to 1:1,000 in the blocking solution

The gradient started with 0% B within the first 2 min and then increased to 80% at 13 min

Copper peptide GHK-Cu is a short chain of amino acids that naturally occurs in the body, found in saliva, urine, and cell plasma

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MASLD consists of different conditions, including isolated liver steatosis (metabolic dysfunction-associated steatotic liver, MASL), metabolic dysfunction associated steatohepatitis (MASH), as well as fibrosis and cirrhosis

Retrieved 22 April 2022, from Zeuschner, C.L., Hokin, B.D., Marsh, K.A., Saunders, A.V., Reid, M.A