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A PBS buffer (pH 7.3) is prepared by dissolving 805.5 mg of sodium chloride (Fisher Scientific), 606 mg of sodium phosphate dibasic anhydrous (Fisher Scientific), 247 mg of sodium phosphate monobasic anhydrous (Fisher Scientific), then QS to 200 g with sterile filtered DI water

based on consistent formation of -helical structure in initial rounds of structure calculations in which no hydrogen bond restraints were defined)

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Was the injection of unlabeled B12 done at the right time

Cohort 2 (from June 2026) covers BMI 35 to 39.9 with four or more conditions