Programmed cell death pathways in the pathogenesis of idiopathic inflammatory myopathies
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[DOI] [PubMed] [Google Scholar] 33.Jin C.-L., Yang L.-X., Wu X.-H., Li Q., Ding M.-P., Fan Y.-Y., Zhang W.-P., Luo J.-H., Chen Z
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Proc Natl Acad Sci USA (2021) 118(13):e2012748118
Four m-thick longitudinally-oriented sections including metaphysis were deparaffinized and rehydrated, and then processed for hematoxylin and eosin (H&E) (Thermo Scientific), tartrate resistant acid phosphatase (TRAP) staining (Sigma-Aldrich) and immunohistochemical staining using primary antibodies against Osterix (Abcam, 1:200 dilution) or Osteocalcin (Takara, 1:200 dilution)
