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Free Radical Damage and Reproductive Cells Oxidative stress occurs when your body produces more free radicals than it can clear

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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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Due to high sequence similarity and presence of evolutionarily conserved sequence domains ( Figure 8A ), we suggest structural and functional similarity of IIGP1 and the members of the INF-I GTPase family analyzed in this section