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Similarly, for overexpression cell lines, gBlocks (IDT) containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB)

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Julong 732, Adlercreutzia equolifaciens , Slakia isoflavoniconvertens , Slakia equolifaciens , Asaccharobacter celatus , Enterorhabdus mucosicola (Actinobacteria), and Lactococcus garvieae (Firmicutes) [56, 57]

Vaccination The intention of vaccination is to train the immune system to respond to a particular disease-causing organism (pathogen), so that when infection occurs, the immune system can fight the pathogen before serious disease arises

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