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The primers used for RTqPCR are presented in Supplementary Table 10

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That said, a significant die-off reaction can also be a signal that detoxification pathways need more supportmore on this below

In contrast to fat-soluble antioxidants, which tend to accumulate in lipid membranes, water-soluble antioxidants like glutathione are more readily available to combat oxidative stress in various cellular environments

These data could all point to a centrally activated cortisol production, were it not that pituitary ACTH levels were low, and circulating levels never increased