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The medication vial was loose in a ziplock bag with air bubbles inside, as though it had been shaken

Western blot analysis Tissues or cells were lysed in ice-cold radio-immunoprecipitation assay (P0013B, RIPA) buffer containing protease and phosphatase inhibitors (phenylmethylsulfonyl fluoride, ST506, Beyotime, Shanghai, China)

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Clinical guidance for subcutaneous injections explicitly states not to use the inner thigh

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VPA-exposed rats spent significantly less time in the target quadrant compared to controls ( P 0.05 )