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Receptor binding One day before transfection, the COS-7 cells were seeded in 75 cm 2 culture flasks (3 million cells/flask) in DMEM, containing 3.9 g/L NaHCO 3 and supplemented with 10% FBS, 1% l-glutamine, 180 units/mL penicillin and 45 g/mL streptomycin