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Calculating the pI of the recombinant protein is also necessary because it can be used to tune the buffer pH for ion exchange chromatography [22]

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2 Applications S-Acetyl-L-glutathione is a modified form of the natural antioxidant molecule glutathione

4,5,6 NADPH is also well known as an essential electron donor and an indispensable cofactor that is used for transferring and reserving reduction potential for numerous anabolic reactions