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Furthermore, using contrast variation, it was shown that the nucleosome unfolding induced by a decrease in ionic strength represents a complex process, where DNA became partially unfolded below the transition point, whereas only small conformational changes occur in the protein core (Uberbacher et al., 1983)

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DALYs and mortality related to obesity are the consequence of the multiple comorbidities spanning from cardiovascular (CV) and metabolic diseases, such as heart failure (HF) and type 2 diabetes (T2D), to many cancers, infertility and respiratory (e.g., obstructive apnea syndrome), cutaneous (e.g