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The surface was preconditioned with 350mM ethylenediaminetetraacetic acid (EDTA) and running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 50 M EDTA, 0.05% Tween-20) prior to loading with 5mM Ni2+

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This is because some people with more weight experience hormonal imbalances and abnormal ovulation, making it more difficult to get pregnant, says Dr

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