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Pen Dosage Chart Dosage & Protocols Variations Standard Research Protocol Dose: 1.0 5.0 mg (= 20100 clicks) Duration: 3 consecutive days Frequency: Once every 2 weeks (intermittent pulse) Cycle Interval: 2 4 weeks off before repeating Goal / Description: Baseline senolytic pulsing observed in preclinical aging models

Metabolite analysis For metabolite analysis of cultured cancer cells, cells were seeded in 6-well plates and treated as specified

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Cell population enrichment across DVC areas was evaluated using Fishers exact tests with Bonferroni correction

4.2 Consequence of the predicted mode of action of the thiol switch in GSAAT1 Structural insights into GSAAT that led us to speculate about the mechanism of action of the thiol switch prompts other questions, which we would like to discuss

FAK-Paxillin Pathway: It activates the focal adhesion kinase (FAK) pathway, which is essential for cell migration and attachment, allowing repair cells to anchor to the site of damage and begin rebuilding