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A single colony was inoculated into 5 ml of Tryptic Soy Broth (BD Difco) under anerobic conditions at 37 C for an overnight culture lasting 3 days

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For electroporation, safety concerns such as erythema, mild pain, and muscle contractions are noted

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We previously proved that inhibiting activity of lysyl oxidase (Anti-lysyl oxidase, Anti-LOX) combined with vacuum erectile device (VED) lengthened penis by remodeling tunica albuginea

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