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Although maintaining coffee brews at a high temperature (like on a hot plate) reduces chlorogenic acid and lactone concentrations, they are still present in relatively high amounts in medium-roasted coffees compared with most food sources (Clifford 2000)
For maximum anti-agin
The p -value calculated with EXCEL (Microsoft, Redmond, WA) using a t -test was used to determine which treatment group was statistically different from the vehicle or negative control
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