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Pixel quantification of images was achieved using Image J and statistics were performed using Prism 5 and InStat v.3.05 (GraphPad

Rhoads JM, Wu G (2009) Glutamine, arginine, and leucine signaling in the intestine

Can Teenagers and Children Take Glutathione

After digestion with restriction enzymes, the PCR product is ligated into the pGEX vector and transformed into an E

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Tetramethylbenzidine (TMB) substrate was then applied and incubated for 30 min, followed by the addition of the stop solution, and absorbance was read at 450 nm using a microplate reader (IRE 96 SFRI plate reader, France)