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HPLC testing: High-performance liquid chromatography confirms peptide identity and purity

This symptom occurs because the vitamin plays a In the nervous system, vitamin B12 helps produce a substance called myelin that shields the nerves and helps them transmit sensations

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The resulting pellet was re-suspended in minimal 50 mM HEPES pH 8.0, 200 mM NaCl, applied to a 1545% w/v sucrose gradient and subjected to ultracentrifugation at 4 C for 3 h, 105,000 g

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