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The plasmid for expressing GST-Sirt4 was prepared as follows: a cDNA encoding residues 29314 of human Sirt4 was cloned into the BamHI and SalI sites of the pGEX 6p-1 vector to obtain the corresponding construct

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Notably, injection site discomfort with GHK-Cu tends to be more frequent than with other peptides, due to the copper component