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In addition, the pretreatment with Ab extract leads to a complete control of bacteremia 24 h post infection (Figure 2G), associated with an augment in NO (Figure 2H), and a significant decrease in IL-1 (Figure 2I) and TNF- (Figure 2J) levels in septic animals

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For SDS-PAGE, the proteins in the Affi-eluates (1020 L) were reduced by -mercaptoethanol or sodium dithionite (DTT, 100 mM), then separated by SDS-PAGE (8% gels), and transferred to PVDF membranes (0.2 micron)

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Br J Dermatol 2009